Program

The course will provide intensive, hands-on training in several of the techniques required to analyze protein-RNA interactions. Participants will briefly introduce themselves and their projects of interest during the first day, with a 5 min. talk. The participant/monitor ratio will be low to promote discussions and provide a platform for questions and in-depth understanding of the topic.

In a daily seminar, an invited speaker will present an overview of his/her field, highlighting recent technical advances as well as the current work from their laboratory. Participants will have the opportunity to meet the speakers during a evening tapas hour.

Topics covered by invited speakers:

• RNA structure
• RNA-protein interactions
• RNP complexes
• pre-mRNA splicing

Topics covered in the practical course:

• In vitro splicing assays
• In vivo (cell culture) splicing
• CLIP: determining RNA targets of proteins in vivo
• analysis of protein-RNA binding in vitro
• in vitro transcription of RNA
• semi-quantitative and quantitative RT-PCR