The sequencing field has experienced a major shift with the introduction of innovative, high-throughput sequencing technologies developed by Solexa/Illumina and 454/Roche. The Ultrasequencing Unit at the CRG is presently equipped with two Illumina Genome Analyzer IIx (GA IIx) sequencers, one Illumina HiSeq2000 sequencer and one Roche 454 FLX sequencing instrument.
Sequencing service is offered to the CRG, PRBB, and external users. We perform ChIP-Seq, mRNA-Seq, sequencing of small RNA molecules such as miRNA, amplicon sequencing, genome re-sequencing, and de novo sequencing.
Starting from DNA or RNA samples provided to us, the service includes:
1. Sequencing library preparation incl. attachment of platform-specific adapters
2. Library quality control
3. Library quantification
4. Performing the sequencing run
5. Basecalling data, sequence data quality control, making data available (sequence files, quality files, alignment files)
In addition to providing next-generation sequencing services, the Unit performs its own research.
More information on the Unit and its members can be found here.