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Advanced Light Microscopy Unit

Unit Head:



Timo Zimmermann
Head of the Advanced Light Microscopy Unit
CRG-Centre for Genomic Regulation
Room 552
Tel. +34 93 316 03 72
Short CV

2000 PhD in Biology at the Ludwig-Maximilians-University, Munich
2000 EMBO Short Term Fellowship at the European Molecular Biology Laboratory, Heidelberg
2001-2006 Senior Technical Officer at the Advanced Light Microscopy Facility, EMBL, Heidelberg
2007- Head of the Advanced Light Microscopy Unit at the Centre for Genomic Regulation, Barcelona
 


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Overview

It is the aim of this unit to provide instruments covering the whole application spectrum of advanced light microscopy and to make them available to researchers from the CRG as well as for visitors from other research institutions.

As Core Facility for light microscopy, the unit provides a number of advanced light microscopy systems, equipment for sample preparation and maintenance prior to imaging and resources for the subsequent processing of the image data.

The staff assists researchers in the experimental planning of light microscopy experiments and provides the in-depth training for the operation of the microscopes and for specific imaging techniques. Additionally, support is provided in the processing, rendering and analysis of the acquired datasets. If needed, custom analysis routines will be designed.

The Head of the Service is Timo Zimmermann. For any  further information, please click here.

AVAILABLE APPLICATIONS

Super-resolution microscopy (Stimulated emission depletion, STED)

Two photon microscopy

Confocal microscopy

Total Internal Reflection Fluorescence (TIRF) microscopy

Fluorescence Lifetime Imaging Microscopy

Fluorescence Correlation Spectroscopy

Automated widefield imaging

Environmental control for in-vivo imaging:

Microinjection

Microdissection

Image processing and analysis

Selected Publications

von Blume J, Alleaume AM, Cantero-Recasens G, Curwin A, Carreras-Sureda A, et al.
ADF/Cofilin Regulates Secretory Cargo Sorting at the TGN via the Ca(2+) ATPase SPCA1.
Dev Cell 20(5):652-62 (2011). PubMed ID: 21571222.abstract
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